500 uM IAA 6h, 3 uM STI-571 with 500uM IAA treated for 4 days
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked cells were treated with cell lysis buffer (5mM PIPES pH 8, 85mM KCl, 0.5% NP-40) and nuclei lysis buffer (50mM TrisCl pH 8.1, 10mM EDTA, 1% SDS) and subjected to sonication. IP was performed using Rad21 antibody(Abcam, #ab992) or CTCF antibody (Millipore, #07-729). IP and Input DNA were de-crosslinked at 65o overnight and purified via Qiagen PCR purification columns. ChIP-Seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB, #E7645)