GSM1537294: RNAPolII-WI38 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Lung
Cell type
WI-38
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Human Embryonic Lung Fibroblasts
gender
female
cell line
WI38
cell type
Normal Cells
antibody
Santa Cruz; sc-9001; H-224
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Phenol/chlorophrom extraction was used for gDNA isolation. Standard Illumina protocols are followed. ChIP-seq: Bioo Scientific NEXTflex kit is used (Catalog: 514120, 48 reactions) ChIP-seq: Libraries were prepared according to the standard Illumina protocol and sequenced with the HiSeq2500 system. Single end sequencing was carried out to obtain around 25-150 million (M), 100bp long reads per sample.