Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Breast

Cell type

MDA-MB-436

Primary Tissue

Breast

Site of Extraction

Effusion, Pleural

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Human Breast Cancer Cells

gender

female

cell line

MDAMB436

cell type

Basal-Like Carcinoma

antibody

Abcam; ab8580

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Phenol/chlorophrom extraction was used for gDNA isolation. Standard Illumina protocols are followed. ChIP-seq: Bioo Scientific NEXTflex kit is used (Catalog: 514120, 48 reactions) ChIP-seq: Libraries were prepared according to the standard Illumina protocol and sequenced with the HiSeq2500 system. Single end sequencing was carried out to obtain around 25-150 million (M), 100bp long reads per sample.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

39002033

Reads aligned (%)

98.8

Duplicates removed (%)

42.3

Number of peaks

23659 (qval < 1E-05)

hg19

Number of total reads

39002033

Reads aligned (%)

98.1

Duplicates removed (%)

43.5

Number of peaks

23303 (qval < 1E-05)