Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549 lung cancer cell line
cell line
A549 lung cancer cell line
chip antibody
EZH2 (Cell Signaling Technology,#5346)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation and DNA extraction were performed using SimpleChIP Enzymatic Chromatin IP Kit (9003, Cell Signaling) following manufacturer's instructions. ChIP-seq libraries were prepared with TruSeq DNA sample prep kit using standard protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
85055614
Reads aligned (%)
167.3
Duplicates removed (%)
12.2
Number of peaks
591 (qval < 1E-05)

hg38

Number of total reads
85055614
Reads aligned (%)
169.8
Duplicates removed (%)
11.7
Number of peaks
1737 (qval < 1E-05)

Base call quality data from DBCLS SRA