PROTOCOL1: All RNA samples were examined for quantity and quality by NanoDrop and Bioanalyzer (Agilent). Samples were randomized for RNA extractions and sequencing. 2ug of total RNA were treated with Ribo-zero Removal Kit according to the manufacturer’s instructions to remove ribosomal RNA. PROTOCOL2: DNA was purified using Qiagen MinElute Columns and quantified using a Qubit Fluorometer according to the manufacturer’s instructions. PROTOCOL3: All RNA samples were examined for quantity and quality by NanoDrop and Bioanalyzer (Agilent). Samples were randomized for RNA extractions and sequencing. 2ug of total RNA were treated with Ribo-zero Removal Kit according to the manufacturer’s instructions to remove ribosomal RNA. Libraries were prepared using an Illumina TruSeq kit according to the manufacturer’s instructions.