Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

cdc_k4me3

cell type

moDC

tissue

Bone marrow

strain

C57BL/6

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixated, lyzed and nuclei were sonicated. Sonicated nuclei were mixed with protein G magnetic dynabeads coupled to target antibbody. Libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

16767021

Reads aligned (%)

98.2

Duplicates removed (%)

10.7

Number of peaks

23200 (qval < 1E-05)

mm9

Number of total reads

16767021

Reads aligned (%)

98.0

Duplicates removed (%)

10.8

Number of peaks

23228 (qval < 1E-05)