Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESC

chip antibody

H3K27me3

library strategy

ChIP-seq

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed according to standard protocols as described previously (Ferrari et al., 2014) DNA libraries were prepared with 2–10 ng of DNA using an in-house protocol (Blecher-Gonen et al., 2013) by the IEO genomic facility

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

31659183

Reads aligned (%)

93.4

Duplicates removed (%)

14.4

Number of peaks

196 (qval < 1E-05)

mm9

Number of total reads

31659183

Reads aligned (%)

93.3

Duplicates removed (%)

14.5

Number of peaks

182 (qval < 1E-05)