Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Lung

Cell type

A549

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma

Sample

source_name

A549 CELLS

cell line

A549

cell type

lung adenocarcinoma cells

passages

3 to 5

ip reagent

α-APE1; Mouse monoclonal (Novus biologicals #13B8E5C2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei. ARP-tagged DNA was isolated with Streptavidin and DNA-protein complexes were isolated with antibody. The library was made using New England Biolabs NEB Next Ultra II DNA Library Prep Kit. High-throughput sequencing was conducted at the UNMC Sequencing Core, using an Illumina HiSeq 2500 Genome Analyzer.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

40002707

Reads aligned (%)

89.5

Duplicates removed (%)

6.1

Number of peaks

3666 (qval < 1E-05)

hg19

Number of total reads

40002707

Reads aligned (%)

89.0

Duplicates removed (%)

6.6

Number of peaks

3546 (qval < 1E-05)