Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Spermatocytes

MeSH Description

Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

Sample

source_name

mouse pachytene spermatocytes

background strain

C57BL/6J

chip antibody

none

genotype

WT

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq analysis, total RNA from ES cells on each well of 24 well plate was extracted using an RNeasy Plus Mini Kit (QIAGEN), with gnomic DNA elimination. For ChIP-seq analysis, to extract chromatin, isolated pachytene spermatocytes were suspended in 20 µl of Nuclei EZ Lysis Buffer (SIGMA), and extensively digested chromatin with 2 IU/µl Micrococcal Nuclease (MNase, NEB) at 37℃ for 5 min. RNA-seq library preparation was carried out using TruSeq Stranded mRNA Library Prep kit (Illumina) following manufacturer's instructions. ChIP-seq library preparation was carried out by NEBNext Ultra II DNA Library Prep Kit (NEB) following manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

40990729

Reads aligned (%)

97.3

Duplicates removed (%)

7.1

Number of peaks

182 (qval < 1E-05)

mm9

Number of total reads

40990729

Reads aligned (%)

97.2

Duplicates removed (%)

7.2

Number of peaks

157 (qval < 1E-05)