Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Spermatocytes

MeSH Description

Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

Sample

source_name

Testicular cells

strain

C57BL6

cell type

Pachytene cells

age

post natal day 20

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin Immunprecipitation were carried using the published protocol (Tardar et al., 2010). Briefly, chromatin were extracted from P20 mouse testicular cells, sonicated for 40 cycles, set for overnight antibody binding at 4 degrees. The dynabeads were added the next day and washes were given with multiple wash buffers. DNA were extracted from the elute fractions using the phenol-choloroform method. The input and ChIP DNA libraries were prepared using the NEBNext Ultra II DNA library preparation kit.

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

27956943

Reads aligned (%)

89.5

Duplicates removed (%)

41.6

Number of peaks

275 (qval < 1E-05)

mm9

Number of total reads

27956943

Reads aligned (%)

89.3

Duplicates removed (%)

42.2

Number of peaks

241 (qval < 1E-05)