Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Larvae

Cell type

Eye-antennal disc

NA

NA

Sample

source_name

eye-antennal discs

tissue

eye-antennal discs

developmental stage

third instar larvae

sample extraction method

Hand dissection

genotype

grh-GFP.FPTB}VK00033

antibody

anti-GFP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated chromatin from eye antennal discs and Grh-GFP-DNA complexes were isolated with a GFP antibody. Libraries were prepared according to Illumina's instructions accompanying the Trueseq DNA library prep.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

5500179

Reads aligned (%)

97.7

Duplicates removed (%)

30.2

Number of peaks

7934 (qval < 1E-05)

dm3

Number of total reads

5500179

Reads aligned (%)

98.3

Duplicates removed (%)

30.5

Number of peaks

7954 (qval < 1E-05)