GSM4217624: MG4H H3K4me3 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF-7_MG4H_H3K4me3
cell line
MCF-7
cell type
breast cancer cells
treatment
MG132 (4H)
chip antibody
H3K4me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MNase digested ChIP DNA was collected from MCF7 cells cultured in phenol red free MEM, supplemented with charcoal stripped serum and containing 0.1% DMSO or 1 uM MG132 for 4 or 24H. Cells were incubated for 4 or 24 hours at 37 ºC in a humidified incubator with 5% CO2. NEXTFlex Rapid DNA-Seq Kit BIOO Scientific Cat#5144-02