Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Neural
Cell type
90-8TL
NA
NA

Attributes by original data submitter

Sample

source_name
MPNST cells 90-8TL_PDJQ
cell line
MPNST cell line 90-8TL
treated with
PD-032901 (2uM) + JQ1 (100nM) for 24hrs
chip antibody
BRD4
chip antibody vendor
Bethyl

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPed following the Agilent Mammalian ChIP-on-chip protocol. Cells were cross- linked for 15 min at room temperature by the addition of one-tenth of the volume of 11% formaldehyde solution to the growth media. NEBNext ChIP Master Mix Kit for Illumina following manufacturers instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16034053
Reads aligned (%)
67.8
Duplicates removed (%)
27.9
Number of peaks
12403 (qval < 1E-05)

hg38

Number of total reads
16034053
Reads aligned (%)
69.7
Duplicates removed (%)
26.4
Number of peaks
12360 (qval < 1E-05)

Base call quality data from DBCLS SRA