Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

LSK cells

NA

NA

Sample

source_name

LSK_HEME_28d_scATAC-seq

strain

C57BL/6

treatment

2 mg/kg Heme (pH 7.4) i.p.

tissue

Bone Marrow (BM)

bm sampling/sorting time point

28 days later

cell type

LSK cells

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Nuclei were isolated with a mix of detergents (Digitonin, Tween and NP40). Up-front tagmentation and single-nuclei partition into GEMs were performed using the Chromium Controller and 10x Genomics reagents. Libraries were generated using the scATAC-seq reagent kit from 10x Genomics

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

73656068

Reads aligned (%)

97.5

Duplicates removed (%)

0.0

Number of peaks

442 (qval < 1E-05)

mm9

Number of total reads

73656068

Reads aligned (%)

97.5

Duplicates removed (%)

0.0

Number of peaks

432 (qval < 1E-05)