Antigen

Antigen Class

TFs and others

Antigen

YY1

Cell type

Cell type Class

Liver

Cell type

PLC/PRF/5

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

YY1-ChIP Met

cell line

PLC-PRF-5

treatment

600μM metformin for 48h

chip antibody

YY1 (Abcam, ab109237)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Twist1-DNA or YY1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

23188365

Reads aligned (%)

39.5

Duplicates removed (%)

10.1

Number of peaks

224 (qval < 1E-05)

hg19

Number of total reads

23188365

Reads aligned (%)

39.2

Duplicates removed (%)

10.2

Number of peaks

138 (qval < 1E-05)