Antigen

Antigen Class

TFs and others

Antigen

TWIST1

Cell type

Cell type Class

Liver

Cell type

PLC/PRF/5

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

Twist1-ChIP Control

cell line

PLC-PRF-5

chip antibody

Twist1 (Abcam, ab50887)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Twist1-DNA or YY1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

31792480

Reads aligned (%)

38.3

Duplicates removed (%)

13.5

Number of peaks

460 (qval < 1E-05)

hg19

Number of total reads

31792480

Reads aligned (%)

38.0

Duplicates removed (%)

13.7

Number of peaks

240 (qval < 1E-05)