GSM4215606: hela-siNC-repli-seq S2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Hela cell
cell line
Hela cell
genotype
siNC
chip antibody
BD, BD44, S2
library strategy
ChIP-seq
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were first crosslinked by formaldehyde, then sonicated in SDS containing buffer to dissolve the chromatin. The clarified chromatin were immunoprecipitated with dyno beas conjucated with antibodies. For ORC1 ChIP, the chromatin were immunoprecipitated with streptavidin dyno beads( Invitrogent, M280). For native ChIP-seq, cells were collected and digested by MNase to get the mono-nucleosome. For BrdU-IP-seq, genomic DNA were extracted from cells and then sonicated to 200-500bp. The ChIP-seq antibodies are as following: Anti-H2A.Z, Abcam, ab4174; Anti-BrdU, BD, BD44; Anti-H4K20me1, Abcam, ab9051; Anti-H4K20me2, Abcam, ab9052; Anti-H4K20me3, Abcam, ab9053. H2A.Z, H4K20me, ORC1,BrdU Libraries(sample 1-22, 35-44, 49-52) were prepared according to NEBNext Ultra DNA Library Prep Kit for Illumina (E7370L) and were sequenced using HiSeq2000 or Nova seq.