Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

breast cancer cells

cell line

T47D

growth medium

Full media

chip antibody

H3K27ac(Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. The extracted ChIP DNA was ligated to specific adaptors using KAPA Hyper Prep Kit (KK8504), according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

17976242

Reads aligned (%)

96.8

Duplicates removed (%)

21.2

Number of peaks

27048 (qval < 1E-05)

hg19

Number of total reads

17976242

Reads aligned (%)

96.6

Duplicates removed (%)

21.4

Number of peaks

26991 (qval < 1E-05)