Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
V6.5
chip antibody
H3K9me3
antibody catalog
ab8898
young_id
20140829_3000
system
Stem Cell

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified. Lysates were clarified from sonicated chromatin fraction and target bound fragments were isolated with antibody

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
45604120
Reads aligned (%)
86.6
Duplicates removed (%)
20.9
Number of peaks
775 (qval < 1E-05)

mm9

Number of total reads
45604120
Reads aligned (%)
86.2
Duplicates removed (%)
20.8
Number of peaks
888 (qval < 1E-05)

Base call quality data from DBCLS SRA