Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TEAD1

Cell type

Cell type Class
Lung
Cell type
NCI-H69
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Small Cell

Attributes by original data submitter

Sample

source_name
H69 human cell line
cell line
H69
chip antibody
TEAD

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 10 min and cross-linking was quenched by addition of 0.125 M glycine. Cells were then harvested in ChIP buffer and sonicated with a Bioruptor UCD-300 to obtain fragments around 250 bp in size. Precipitated DNA was recovered by purification with a QIAquick PCR purification kit (Qiagen) Libraries for sequencing were obtained using a ChIP-Seq DNA sample prep kit (Illumina) according to the manufacturer's reccomendations

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
40036304
Reads aligned (%)
32.5
Duplicates removed (%)
62.5
Number of peaks
4867 (qval < 1E-05)

hg38

Number of total reads
40036304
Reads aligned (%)
33.5
Duplicates removed (%)
61.7
Number of peaks
4863 (qval < 1E-05)

Base call quality data from DBCLS SRA