Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

beast cancer cell line

cell line

MCF7

chip antibody

ERa

treatment

cells transfected with siNCAPG, and 1hr with 100nM E2, double crosslinked

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were crosslinked with 1% of formaldehyde or double-crosslinked with DSG first followed by 1% of formaldehyde, clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The extracted ChIP DNA was ligated to specific adaptors of Truseq system from Illumina, according to the manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

16144928

Reads aligned (%)

78.1

Duplicates removed (%)

12.2

Number of peaks

6804 (qval < 1E-05)

hg19

Number of total reads

16144928

Reads aligned (%)

77.5

Duplicates removed (%)

13.3

Number of peaks

6914 (qval < 1E-05)