GSM1522563: KLF9 ChIP GBM1A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Neural
Cell type
HSR-GBM1A
NA
NA
Attributes by original data submitter
Sample
source_name
DNA from glioblastoma stem cells
cell line
GBM1A
cell type
glioblastoma stem cell line
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were subjected to ChIP using MAGnify ChIP system (Invitrogen) following manufacturer’s protocol. KLF9 bound DNA was immunoprecipitated using mouse anti-FLAG M2 antibody (Sigma-Aldrich) and Dynabeads Magnetic Beads (Life Technologies). ChIP-enriched DNA was used for PCR and deep sequencing. ChIP-enriched DNA or input DNA (10ng each) was subjected to library preparation using ChIP-Seq DNA Sample Prep Kit (Illumina) following manufacturer’s protocol.