Antigen

Antigen Class

Histone

Antigen

H2A.XS139ph

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa cells

transfection

N/A

chip antibody

γH2AX (Abcam, ab2893)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixated with 1% formaldehyde for 13 minutes before extracting ChIP DNA using the SimpleChIP plus sonication kit (Cell Signaling Technology) Libraries were prepared using Illumina standard protocols by Genewiz

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

26441674

Reads aligned (%)

94.5

Duplicates removed (%)

8.0

Number of peaks

1091 (qval < 1E-05)

hg19

Number of total reads

26441674

Reads aligned (%)

93.6

Duplicates removed (%)

8.3

Number of peaks

341 (qval < 1E-05)