Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Lung

Cell type

Alveolar Epithelial Cells

MeSH Description

Large flat epithelial cells that line the PULMONARY ALVEOLI and are involved in PULMONARY GAS EXCHANGE.

Sample

source_name

Alveolar epithelial type 2 cells in homeostasis

cell type

RFP-neg alveolar epithelial type 2 cells

group

Mki67-tagRFP

age

3 months

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For sorting AEC2 cells from Mki67-tagRFP mice, cells were stained with CD31, CD45, CD326 and lysotracker then CD31-neg/CD45-neg/CD326-pos/Lysotracker-pos/tagRFP-neg cells were sorted. For sorting CTGFpos cells from CTGF-GFP mice, cells were stained with CD31, CD45, Lgals3, CD326 then CD31-neg/CD45-neg/Lgals3-neg/CD326-pos/GFP-pos cells were sorted. Extraction of DNA, ChIP reactions and library constractions were performed uisng Mint-ChIP3 protocol (https://www.protocols.io/view/mint-chip3-a-low-input-chip-seq-protocol-using-mul-wbefaje)

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

9511147

Reads aligned (%)

67.2

Duplicates removed (%)

13.7

Number of peaks

135 (qval < 1E-05)

mm9

Number of total reads

9511147

Reads aligned (%)

66.9

Duplicates removed (%)

13.7

Number of peaks

132 (qval < 1E-05)