ChIP-Atlas: SRX7283319

Antigen

Cell type Class: Uterus
Cell type: Decidua
MeSH Description: The hormone-responsive glandular layer of ENDOMETRIUM that sloughs off at each menstrual flow (decidua menstrualis) or at the termination of pregnancy. During pregnancy, the thickest part of the decidua forms the maternal portion of the PLACENTA, thus named decidua placentalis. The thin portion of the decidua covering the rest of the embryo is the decidua capsularis.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Multiple pools of freshly dissected decidua (E6.5) from mice expressing an endogenous N-terminal EGFP tagged Blimp1 protein (Mitani et al., 2017) and WT mice were minced using a razor blade in a drop of culture media (RPMI-1640, 10% FCS, 0.0004% β-mercaptoethanol, 1x Pen/Strep, 2mM glutamine) and cross-linked in 1% formaldehyde in culture media for 20 min at ambient temperature. After quenching in 0.125 M glycine for 5 min, cells were washed in PBS and processed for ChIP using 6 micrograms of rabbit anti-GFP antibody (Ab290, Abcam). Triplicate test (GFP ChIP of Prdm1NEG/NEG) and duplicate control (GFP ChIP of WT) decidual samples, and their respective input samples were analysed. DNA was multiplexed and paired end sequencing was performed over two lanes of Illumina HiSeq4000 platform.