GSM4205827: w ChIP R3; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Embryo
Cell type
Embryos
NA
NA
Attributes by original data submitter
Sample
source_name
embryo
maternal_genotype
w
sample_genotype
control_ChIP
timepoint
blastoderms
Sex
NA
experiment
3
collection_temp
NA
library_prep_date
2019-02-19
pcr_cycles
15
library_concentration_ng_ul
4.88
raw_reads_both_pairs
20383874
map_rate
0.69
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ATAC samples were processed as described previously (Blythe and Wieschaus, eLife 2016 Nov 23;5. pii: e20148. doi: 10.7554/eLife.20148.). ChIP samples were processed as described previously (Blythe and Wieschaus, Cell 2015 Mar 12;160(6):1169-81. doi: 10.1016/j.cell.2015.01.050) ATAC libraries were made using the Nextera Tn5 transposase as described in the cited protocol. ChIP libraries were made using the NEBNext Ultra II Library prep kit.