Antigen

Antigen Class

TFs and others

Antigen

TCF4

Cell type

Cell type Class

Bone

Cell type

A-673

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

source_name

Ewing sarcoma cell line

cell line

A673

cell type

Ewing sarcoma cell line

chip antibody

anti-TCF4

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNAs were isolated using miRNeasy RNA isolation kit (Qiagen). RNA sequencing libraries were prepared using TruSeq Library Prep Kit (Illumina) according to the manufacturer's protocol, and were sequenced on HiSeq sequencer (Illumina). A673 cell lysates were clarified from sonicated nuclei and TF-DNA complexes were isolated with following antibody: Anti-KLF15 (Proteintech, 66185-1-Ig), anti-TCF4 (Abcam, ab223073), anti-NKX2-2 (Proteintech, 13013-1-AP). Immunoprecipitated DNA was extracted with the Min-Elute PCR purification kit (Qiagen), followed by DNA library preparation using standard Illumina protocol and sequencing on HiSeq 4000 platform (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

20760789

Reads aligned (%)

84.0

Duplicates removed (%)

4.0

Number of peaks

11309 (qval < 1E-05)

hg19

Number of total reads

20760789

Reads aligned (%)

83.3

Duplicates removed (%)

4.0

Number of peaks

11018 (qval < 1E-05)