GSM4204802: A673 KLF15 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
KLF15
Cell type
Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma
Attributes by original data submitter
Sample
source_name
Ewing sarcoma cell line
cell line
A673
cell type
Ewing sarcoma cell line
chip antibody
anti-KLF15
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNAs were isolated using miRNeasy RNA isolation kit (Qiagen). RNA sequencing libraries were prepared using TruSeq Library Prep Kit (Illumina) according to the manufacturer's protocol, and were sequenced on HiSeq sequencer (Illumina). A673 cell lysates were clarified from sonicated nuclei and TF-DNA complexes were isolated with following antibody: Anti-KLF15 (Proteintech, 66185-1-Ig), anti-TCF4 (Abcam, ab223073), anti-NKX2-2 (Proteintech, 13013-1-AP). Immunoprecipitated DNA was extracted with the Min-Elute PCR purification kit (Qiagen), followed by DNA library preparation using standard Illumina protocol and sequencing on HiSeq 4000 platform (Illumina).