GSM4195003: FANCD2+APH ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FANCD2
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 cells
cell type
human colon carcinoma cell line ATCC
chip antibodies
NB100-182 Novus
cell line
HCT116
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde for 15 min, then quenched in 125mM glycine. After cell lysis, 30 µg of chromatin was used for immunoprecipitation using a FANCD2 antibody (Novus, NB100-182). Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Libraries were prepared according to Active Motif proprietary protocols.