Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Neural

Cell type

Adult brains

NA

NA

Sample

source_name

human brain gDNA

technology

WGA-BS

dna amount

10 pg

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

DNA was purified using Qiagen's Puregene kit and sonicated to about 200 bp. Methylated adapters were added with the NEBNext DNA library prep kit. Libraries were bisulfite converted with the Zymo EZ DNA Methylation Lightning kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

30284570

Reads aligned (%)

17.3

Coverage rate (×)

0.0

Number of hyper MRs

2324 (qval < 1E-05)

hg19

Number of total reads

30284570

Reads aligned (%)

17.3

Coverage rate (×)

0.0

Number of hyper MRs

2453 (qval < 1E-05)