Antigen

Antigen Class

TFs and others

Antigen

Tet1

Cell type

Cell type Class

Gonad

Cell type

Spermatogonia

MeSH Description

Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.

Sample

source_name

mouse testes spermatogonia cells

strain

129

cell type

spermatogonia cells

genotype/variation

Tet1 knock out

antibody

Tet1-antibody

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

we use 20000 cells by FACS and incubate with Tet1-antibody. DNA libraries were prepared for sequencing using standard protocols.

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

6951989

Reads aligned (%)

62.9

Duplicates removed (%)

19.0

Number of peaks

78 (qval < 1E-05)

mm9

Number of total reads

6951989

Reads aligned (%)

62.8

Duplicates removed (%)

18.9

Number of peaks

59 (qval < 1E-05)