Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa B2 cells
cell line
HeLa
treatment protocol
1µM of Triamcinolonacetonide (T6501, Sigma-Aldrich) with 10ng/ml Tumor necrosis factor a (T0157, Sigma-Aldrich) for the last 1 hour.
chip antibody
H3K4me3 (C15410003, Diagenode)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
26370286
Reads aligned (%)
97.7
Duplicates removed (%)
27.2
Number of peaks
24326 (qval < 1E-05)

hg19

Number of total reads
26370286
Reads aligned (%)
97.4
Duplicates removed (%)
27.6
Number of peaks
24307 (qval < 1E-05)

Base call quality data from DBCLS SRA