GSM4175883: GFP ChIP in 2C-like (GFP+) cells from Zprom::GFP mouse ESC reporter line; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GFP
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
E14 mouse ESC with Zscan4c promoter reporter driving expression of GFP
cell line
E14Tg2a
cell type
Embryonic stem cells (ESC)
genotype/variation
Zscan4c promoter reporter driving expression of GFP
cell population
GFP+ (2C-like cells)
chip antibody
GFP (ab290, Abcam)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ChIP-DNA complexes were isolated with antibody. After reverse-crosslinking, input and ChIP DNA were purified by phenol:chloroform extraction. Sequencing libraries were prepared starting from ~30ng of input or ChIP DNA with NEBNext Multiplex Oligos for Illumina kit.