GSM4175875: Input control in 2.5μM curaxin treated mouse ESC replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
E14 mouse ESC
cell line
E14Tg2a
cell type
Embryonic stem cells (ESC)
treatment
2.5uM Curaxin
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ChIP-DNA complexes were isolated with antibody. After reverse-crosslinking, input and ChIP DNA were purified by phenol:chloroform extraction. Sequencing libraries were prepared starting from ~30ng of input or ChIP DNA with NEBNext Multiplex Oligos for Illumina kit.