Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

bone marrow-derived macrophages

strain

C57BL/6

age

8 weeks

genotype

Control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as described previously (Steger et al., 2008) , using HDAC3 (abcam7030), H3K27Ac (Abcam 4729), ATF2 (Cell Signaling,D4L2X), ATF3 (Cell Signaling,D2Y5W) ,p65 (Cell Signaling,D14E12), NCoR1 (ell Signaling, E4S4N), NCoR2 (Millipore AntiSMRTe 06-981) antibodies ChIP DNA was prepared for sequencing according to the amplification protocol provided by Illumina

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

36543336

Reads aligned (%)

96.7

Duplicates removed (%)

48.3

Number of peaks

340 (qval < 1E-05)

mm9

Number of total reads

36543336

Reads aligned (%)

96.5

Duplicates removed (%)

48.3

Number of peaks

349 (qval < 1E-05)