Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Uterus

Cell type

HEC-1-A

Primary Tissue

Uterus

Site of Extraction

Endometrium

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HEC-1-A

genotype

METTL3_KD

chip-seq

rabbit anti-H3K4me3 (ab8580, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed using Auto iDeal ChIP-seq kit for Histone (diagenode) with spike-in Chromatin (Active Motif) and spike-in Antibody (Active Motif) following the manufacturer's protocols. Library preparation was performed by using KAPA HyperPlus Kits (Kapabiosystems) according to the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

44332774

Reads aligned (%)

95.4

Duplicates removed (%)

21.9

Number of peaks

24204 (qval < 1E-05)

hg19

Number of total reads

44332774

Reads aligned (%)

95.1

Duplicates removed (%)

22.1

Number of peaks

24093 (qval < 1E-05)