Lineage negative, Sca1 positive, c-Kit positive hematopoietic bone marrow progenitors
tissue
Lineage negative, Sca1 positive, c-Kit positive hematopoietic bone marrow progenitors
weeks after recombination
4
sorting strategy
Lin-;Sca1+;c-Kit+
genotype/variation
Mx1-Cre Tet2 fl/fl
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Bone marrow (BM) cells were isolated into cold phosphate-buffered saline (PBS), without Ca2+ and Mg2+, and supplemented with 2% bovine serum albumin (BSA) to generate single cell suspensions. Red blood cells (RBCs) were removed using ammonium chloride-potassium bicarbonate (ACK) lysis buffer, resuspended in PBS/2% BSA, and filtered through a 40μm cell strainer. Cells were sorted and inmediately underwent the indicated protocol.