GSM4172935: MKL-1 Control-2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Epidermis
Cell type
MKL-1
Primary Tissue
Skin
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
MKL-1 MCC Cells
cell type
MCC tumor derived cell line
cell line
MKL-1
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were washed with PBS twice to achieve >85% viable cells in pellets. ATAC-seq protocol was modified from Corces et al. The transpose reaction was performed using TDE1 Tn5 transposase (15027865; Illumina) and TD buffer (15027866; Illumina). The reactions were cleaned up using MinElute PCR Purification Kit (28004; Qiagen) and amplified using NEBNext High-Fidelity 2X PCR Master Mix (M0541S; New England Biolabs Inc.) with a total of 7 cycles. The libraries were cleaned up using MinElute PCR Purification Kit and additionally with AMPure XP beads (Beckman Coulter) to remove primer dimers and large fragments.