Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

wild-type

strain

C57BL6

genotype

wild-type

tissue

CD4 T cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Polymerase II-DNA complexes were isolated with antibody. At least 1ng of ChIP DNA was used to prepare sequencing library with Illumina ChIP Sample Library Prep Kit (Illumina, USA) followed by 12 cycles of PCR amplification. Barcoded libraries from different samples were pooled together and sequenced on Illumina HiSeq2000 platform in paired-end sequencing runs.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

53242726

Reads aligned (%)

90.0

Duplicates removed (%)

25.1

Number of peaks

7114 (qval < 1E-05)

mm9

Number of total reads

53242726

Reads aligned (%)

89.9

Duplicates removed (%)

25.9

Number of peaks

7115 (qval < 1E-05)