Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

Trim28 KO

strain

C57BL6

genotype

Trim28 KO

tissue

CD4 T cells

chip antibody

sc-899 Pol II (N-20) lot number H3115

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Polymerase II-DNA complexes were isolated with antibody. At least 1ng of ChIP DNA was used to prepare sequencing library with Illumina ChIP Sample Library Prep Kit (Illumina, USA) followed by 12 cycles of PCR amplification. Barcoded libraries from different samples were pooled together and sequenced on Illumina HiSeq2000 platform in paired-end sequencing runs.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

11158199

Reads aligned (%)

85.8

Duplicates removed (%)

17.3

Number of peaks

746 (qval < 1E-05)

mm9

Number of total reads

11158199

Reads aligned (%)

85.5

Duplicates removed (%)

19.3

Number of peaks

731 (qval < 1E-05)