GSM4160684: islet ATAC-Seq rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Pancreas
Cell type
Pancreatic islets
NA
NA
Attributes by original data submitter
Sample
source_name
human cadaveric islet
cell type
a mixture of alpha, beta, delta, PP cells
cell line
fully differentiated
tissue
endocrine tissue
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Roughly 50,000 islet cells were used for each ATAC-Seq assay following the protocol described in Buenrostro et al. (2013). Briefly, cell nuclei was isolated using cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). The nuclei pellet was resuspended in the transposase reaction mix; 25 μL 2× TD buffer, 2.5 μL transposase (Illumina) and 22.5 μL nuclease-free water at 37°C for 30 min. Then transposed DNA fragments were purified using the Qiagen MinElute kit and amplified 10-12 cycles using the Nextera (Illumina) PCR primers. Libraries were sequenced on HiSeq4000 platform.