GSM4160020: Rad21 ChIP LCL D R2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Rad21
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
LCL D
parental cell line
mouse ES cells E14Tg2a
strain
129/Ola, XY
genotype/variation
CTCF-AID, Tir1, Flag-LCL-mRuby 2 transgene at Tigre
kit
Nextera
chip antibody
Anti-Rad21 antibody; abcam (ab992)
agent
Dox
time point
24 h.
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted using RNeasy Mini Kit (Qiagen 74104) as per the manufacturer's protocol. ChIP-seq libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA seq libraries were prepared using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines.