Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
CLC ID
parental cell line
mouse ES cells E14Tg2a
strain
129/Ola, XY
genotype/variation
CTCF-AID, Tir1, Flag-CLC-mRuby 2 transgene at Tigre
kit
Nextera
chip antibody
Anti-Flag M2 Magnetic Beads; Sigma (M8823)
agent
IAA and Dox
time point
24 h.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted using RNeasy Mini Kit (Qiagen 74104) as per the manufacturer's protocol. ChIP-seq libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA seq libraries were prepared using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
46736708
Reads aligned (%)
96.6
Duplicates removed (%)
25.5
Number of peaks
16394 (qval < 1E-05)

mm9

Number of total reads
46736708
Reads aligned (%)
96.3
Duplicates removed (%)
25.4
Number of peaks
16301 (qval < 1E-05)

Base call quality data from DBCLS SRA