Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6

age and sex

8-week-old

age sex

male

nutrient state

ad libitum fed

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Liver harvested from anesthetized mouse was snap-frozen in liquid nitrogen. Lysates were clarified from sonicated nuclei and PPARα-genomic DNA complexes were isolated with PPARα antibody. 10 ng of the pooled DNA was then amplified according to ChIP Sequencing Sample Preparation Guide provided by Illumina, using adaptor oligo and primers from Illumina, enzymes from New England Biolabs and PCR Purification Kit and MinElute Kit from Qiagen.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

49980715

Reads aligned (%)

98.8

Duplicates removed (%)

55.0

Number of peaks

1132 (qval < 1E-05)

mm9

Number of total reads

49980715

Reads aligned (%)

98.5

Duplicates removed (%)

55.0

Number of peaks

1132 (qval < 1E-05)