Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Neural

Cell type

Cortical neuron

NA

NA

Sample

source_name

cortical neurons in vitro differentiated for 7 days, treated with Agonist for cannabinoid receptort signaling, replicate 5

biopsy_tissue

brain

Sex

male

age

E18.5

genotype

WT

strain

C57BL/6

treatment

Ago

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells were pelleted and lysed in a buffer containing Digitonin and transposase enzyme. After transposition the DNA was purified. The purified samples were enriched using index primers and purified librareies were quality checked and sequenced on Illumina HiSeq 3000/4000 instruments.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

42957545

Reads aligned (%)

88.3

Duplicates removed (%)

43.8

Number of peaks

5075 (qval < 1E-05)

mm9

Number of total reads

42957545

Reads aligned (%)

88.2

Duplicates removed (%)

43.9

Number of peaks

5087 (qval < 1E-05)