Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E -ER4 cells, treated with E2

treatment

Estradiol 10nM 24hr

cell type

Derived by transducing the Gata1 knock out cell line G1E with virus expressing the hormone-inducible rescue protein GATA1-ER. Induced for 24 hr.

strain

129

developmental stage

model for adult erythropoiesis

antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation of H3K27ac occupied segments using anti-H3K27ac (Abcam Cat# Ab4729) using published methods (Welch et al. 2004) TruSeq ChIP Sample Prep Kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

45479736

Reads aligned (%)

98.0

Duplicates removed (%)

12.2

Number of peaks

20815 (qval < 1E-05)

mm9

Number of total reads

45479736

Reads aligned (%)

97.8

Duplicates removed (%)

12.2

Number of peaks

20823 (qval < 1E-05)