Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

G1E

Tissue

Blood

Lineage

cellLine

Description

Gata1- erythroid progenitor

Sample

source_name

G1E cells, an erythroid progenitor model

treatment

none

cell type

Derived from Gata1 knock out ES cells; immortalized as a model for erythroid progenitors

strain

129

developmental stage

model for adult erythropoiesis

antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation of H3K27ac occupied segments using anti-H3K27ac (Abcam Cat# Ab4729) using published methods (Welch et al. 2004) TruSeq ChIP Sample Prep Kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

30711432

Reads aligned (%)

98.1

Duplicates removed (%)

15.5

Number of peaks

30456 (qval < 1E-05)

mm9

Number of total reads

30711432

Reads aligned (%)

97.8

Duplicates removed (%)

15.5

Number of peaks

30485 (qval < 1E-05)