Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

neonatal skin

strain

C57 BL/6

cell types

keratinocytes

genotype

WT

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Primary mouse keratinocytes were isolated from newborn back skins and expanded in culture under serum-free conditions with keratinocyte growth medium. As detailed in Hu et al. Genes Dev (2016)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

29119368

Reads aligned (%)

98.1

Duplicates removed (%)

14.9

Number of peaks

633 (qval < 1E-05)

mm9

Number of total reads

29119368

Reads aligned (%)

97.8

Duplicates removed (%)

14.9

Number of peaks

661 (qval < 1E-05)