Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Neural
Cell type
SF7761
NA
NA

Attributes by original data submitter

Sample

source_name
whole cell
cell type
SF7761 cell line
cell cycle stage
asynchronization
chip antibody
EZH2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed the same way as ChIP assay, except that DNA was recovered from the protein G complex using elution buffer (10 mM Tris at pH 8.0, 10 mM EDTA at pH 8.0, 1% SDS, 150 mM NaCL, 5 mM DTT). DNA was subsequently purified using Qiagen Mini Elute PCR purification kit. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen #217004). The ChIP DNA libraries were prepared with the Ovation Ultralow DR Multiplex system (NuGEN). RNA-seq libraries were prepared with ovation RNA-seq system v2 kit (NuGEN).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
31846575
Reads aligned (%)
59.5
Duplicates removed (%)
69.1
Number of peaks
284 (qval < 1E-05)

hg38

Number of total reads
31846575
Reads aligned (%)
61.9
Duplicates removed (%)
68.1
Number of peaks
315 (qval < 1E-05)

Base call quality data from DBCLS SRA