Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Muscle

Cell type

Myoblasts

MeSH Description

Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).

Sample

source_name

healthy donor myoblasts

disease state

healthy

cell type

myoblasts

chip antibody

H3K27me3 rabbit Millipore 07-449

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were prepared starting with 20 million cells (Pubmed ID 27863225) Libraries were prepared according to Illumina instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

68103452

Reads aligned (%)

94.7

Duplicates removed (%)

14.7

Number of peaks

1211 (qval < 1E-05)

hg19

Number of total reads

68103452

Reads aligned (%)

94.1

Duplicates removed (%)

16.3

Number of peaks

1354 (qval < 1E-05)